Composite

Part:BBa_K1997018:Design

Designed by: Chushu Zhu   Group: iGEM16_NUDT_CHINA   (2016-10-13)


P+R->GFP10->Zif268->GFP11->RBS->GFP1-9->TER


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 279
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 436
    Illegal AgeI site found at 520
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 608
    Illegal BsaI.rc site found at 340
    Illegal BsaI.rc site found at 671


Design Notes

This part is designed to be a positive control which contains the complete ZFN protein.


Source

This part was synthesized by company.

References